PQ0101
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 | Sigma nitrocellulose membrane (0.3 ng sensitivity) The FLAG-BAP control was used to show how a typical Western blot would be performed with a control. 20 to 0.3 ng of FLAG-BAP was detected on nitrocellulose. No other bands besides FLAG-BAP and Chemichrome IgG (heavy chain) band were detected. |
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PQ0101
Sigma
ProteoQwest™ Colorimetric Western Blotting Kit, TMB Substrate
for Mouse Monoclonal IgG Antibodies
Description
| General description | This kit is designed for 25 mini-gel sized (10 × 10 cm) blots. It is possible to use this kit for as many as 45 blots if half the suggested amount of reagents is used. |
| All of the components of this ProteoQwest kit have been extensively tested and optimized. | |
| ProteoQwest Colorimetric Western Blotting Kit, TMB Substrate, is designed for high sensitivity colorimetric detection of as little as 0.15 ng of protein. The colorimetric reaction occurs directly on the PVDF or nitrocellulose membrane; no darkroom or film is needed. | |
| Legal Information | ProteoQwest is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| storage temp. | 2-8°C |
Safety
| Hazard Codes | T |
| Risk Statements | 61-37/38-41 |
| Safety Statements | 53-23-26-36/37/39-45 |
| RIDADR | UN 3316 9/PG 2 |
Components
| Kit component also available separately | Chemichrome™ Western Control, (Color Molecular Weight Marker) 200 μL C4236 |
| Western Blocker™ Solution, for HRP detection systems 2 x 400 mL W0138 | |
| Kit component only | Tris Buffered Saline with 0.05% TWEEN 20, pH 8 25 packets |
| Anti-mouse IgG HRP-conjugated antibody 75 μL | |
| 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes 100 mL T0565 |
